Dr. Sheikh Tasduq Abdullah

PK-PD and Toxicology Division
CSIR - Indian Institute of Integrative Medicine,Canal Road, Jammu – 180001
Email: stabdullah@iiim.ac.in

Positions Held  
Position Held Date Organization
Senior Scientist  2012 - Present CSIR-IIIM
Scientist  2008 - 2012 CSIR-IIIM
Scientist - B 2006 - 2008 CSIR-IIIM
Honours & Awards  
  • Dr. Ganesh Ram Mukharya Memorial Gold Medal For Ist Class First     in M.Sc. from Dr. H.S. Gour (Sagar) University.

  • National Eligibility Test (NET) Qualified-2001.

  • DCF award by National Institute of Drug Abuse (NIDA)/National Institute of Health (NIH) USA, to attend the 43rd Meeting of TIAFT 2005 held at Seoul, Korea, September, 2005.

  • Regional Representative of South Asian Congress of Forensic Medicine, Forensic Science and Toxicology (SAFCON).

  • International Union of Toxicology (IUTOX), Young Scientist Award (RASS-2008) at Germany.

  • Indian Council of Medical Research Young Biomedical Scientist Fellowship-2009.

  • Council of Scientific and Industrial Research (CSIR), Raman Fellowship 2011-2012.

  • Indo-US Research Fellowship Award- 2012 

Membership  
  • Life Member, The International Association of Forensic Toxicologist (TIAFT).

  • Associate Member, The Cytometry Society, India

  • Member, Society of Toxicology, USA.

  • Member, Society of Toxicology, India (SOT-India).

Research Area

Primary interests are Skin and Liver Biology.
Skin Biology:
The major research focus of our lab is skin photo-damage (Photo-carcinogenesis and Photo-aging), melanogenesis and melanoma. Solar UV radiation is known to elicit plenitude of events which culminates in skin photodamage. This lab studies major events responsible for initiating UVB induced photo-aging and skin cancer. The process of photo-aging is associated with alterations in extracellular matrix (ECM) and involves skin cells, such as keratinocytes, fibroblasts and infiltrating neutrophils. This lab studies the cellular, biochemical and molecular basis of UVB-induced photo-aging, along with the identification of target based potent therapeutic agents. Melanoma, a cancer of melanocytes, is arguably one of the most lethal and therapeutically resistant forms of skin cancer. This lab studies how melanoma and other skin cells interact with natural products, respond to UVB–irradiation and maintain cellular homeostasis. Melanogenesis, a highly complex process of melanin biosynthesis, is one of the most visible physiological responses of skin to UV exposure. Despite certain contradictory goals, an even skin tone is ideal of human beauty and depends on melanogenesis, among other factors.  This lab also focuses on the mechanistic analysis of novel molecules that modulate melanogenesis for achieving therapeutically and cosmetically useful outcomes. A salient feature of UVB-irradiation is generation of reactive oxygen species (ROS) and simultaneous modulation of ER stress, autophagy and apoptosis. This lab is particularly interesting in understanding the mechanistic basis for the UVB induced ROS generation, ER stress, autophagy and apoptosis in skin cells

Liver Biology:
In liver biology we wish to understand the pathophysiology/pathogenesis of Non-alcoholic fatty liver disease and develop therapeutic interventions using novel approaches.
Liver is the major player in the regulation of fat metabolism as it integrates endogenous and exogenous fatty acids. Any defect/perturbation in the liver fat metabolism potentially impacts the functioning of the liver. Lipotoxicity is one such condition which arises due to the perturbed or defective fat metabolism in liver. It starts with the accumulation of fat in the liver, which underlies spectrum of conditions known as Non-alcoholic fatty liver disease (NAFLD). Prevalence of non-alcoholic fatty liver disease (NAFLD) has been on an inclined trajectory with the rise in obesity and has been found to be affecting about 50% of individuals in some populations. Non alcoholic steatohepatitis (NASH), an advanced stage of NAFLD, has emerged as third most common cause of liver transplantation. NAFLD can progress to cirrhosis and liver failure in 3% to 15% cases and there have been emerging reports of a significant proportion of HCC cases in settings of NAFLD. Despite the high prevalence of NAFLD and its serious complications the underling molecular events that implicate lipotoxicity remain poorly understood and need further investigation.
Our research interest focuses on normal and diseased liver pathology. The research specifically focuses on metabolic and lipotoxic changes in the pathogenesis of NAFLD, the mechanism of action of various treatment options and response of In vivo model of disease to the treatment.

The Specific areas of our interest are:

In the area of Skin Biology:

  1. To study the cellular and biochemical basis of UVB induced photodamage.

  2. To understand the mechanistic basis for the photo protective role of natural products in skin cells.

  3. To elucidate the role of natural products in promoting cell death in UVB –irradiated melanoma cells

  4. To understand the mechanistic basis for the melanogeneis modulatory activity of natural products

  5. To understand the role of ER strees, autophagy, and apoptosis in UVB –irradiated skin cells.

In the area of Liver Biology:

  1. Cellular and molecular events of lipotoxicity in hepatocytes.

  2. Roe of unfolded protein response (UPR) and autophagy in the development of Lipid droplet formation and lipotoxicity.

  3. Development of diet based animal models mimicking human NAFLD.

  4. Targeting autophagy and UPR for the treatment of lipotoxic conditions such as NAFLD.

Publications
  • Pathania, A.S.; Guru, S.K.; Ul, Ashraf N.; Riyaz-Ul-Hassan, S.; Ali, A.; Abdullah, T.S.; Malik, F.; Bhushan, S. A novel stereo bioactive metabolite isolated from an endophytic fungus induces caspase dependent apoptosis and STAT-3 inhibition in human leukemia cells. Eur J Pharmacol (2015), 75-85.

  • Nissar, A.U.;  Tasduq, S.A. Endoplasmic reticulum stress and Oxidative stress in the pathogenesis of Non-alcoholic fatty liver disease. Free Radic Res (2015), 1-32.

  • Ashraf-Ul-Nissar.; Sharma, Love.; Tasduq, S.A. Palmitic acid induced lipotoxicity is associated with altered lipid metabolism, enhanced CYP 450 2E1 and intracellular calcium mediated ER stress in human hepatoma cells. Toxicology Research (2015), 1344-1358.

  • Rather, A.R.; Afnan, Quadri.; Lone, A.N.; Kaiser, Peerzada.; Bashir, A.G.; Tasduq, S.A. A Potent Inhibitor of Phosphoinositide 3-Kinase (PI3K) and Mitogen Activated Protein (MAP) Kinase Signalling, Quercetin (3, 3’, 4’, 5, 7-pentahydroxyflavone) Promotes Cell Death in Ultraviolet (UV)-B-irradiated B16F10 Melanoma cells. PLoS one (2015), e0131253.

  • Mufti, R.F.; Ul-Ashraf, Nissar.; Kaiser, J.P.; Quadri, Afnan.; Praduman, R.S.; Bhushan, Shashi .; Tasduq, SA.Glycyrrhizic acid (GA) sinhibits Reactive Oxygen Species mediated photodamage by blocking ER stress and MAPK pathway in UV-B irradiated human skin fibroblasts. Journal of Photochemistry and Photobiology B: Biology (2015), 351–357.

  • Guru, S.K.; Pathania, A.S.; Kumar, S.; Ramesh, D.; Kumar, M.; Rana, S.; Kumar, A.; Malik, F.; Sharma, P.R.; Chandan, B.K.; Jaglan, S.; Sharma, J.P.; Shah, B.A.;Tasduq, SA.; Lattoo, S.K.; Faruk, A.; Saxena, A.K.; Vishwakarma, R.A.; Bhushan, S. Secalonic acid-D represses HIF-1α/VEGF mediated angiogenesis by regulating the Akt/mTOR/p70S6K signaling cascade. Cancer Res (2015), 2886-96.

  • Peerzada, K.J.; Faridi, A.H.; Sharma, Love.; Bhardwaj, S.C.; Satti, N.K.; Shashi, Bhushan.; Tasduq, SA. Acteoside-mediates chemoprevention of experimental liver carcinogenesis through STAT-3 regulated oxidative stress and apoptosis. Enviornmental Toxicology (2015), (Accepted MS).

  • Rather, R.A.; Ganai, B.A.;Tasduq, SA. Piperine promotes ultraviolet (UV)-B-induced cell death in B16F10 mouse melanoma cells through modulation of major regulators of cell survival. RSC Advances(2015), 11884-11894.

  • Dar, A.A.; Sangwan, P.L.; Khan, I.; Gupta, N.; Qaudri, A.; Tasduq, SA.; Kitchlu, S.; Kumar, A.; Koul, S. Simultaneous quantification of eight bioactive secondary metabolites from Codonopsis ovata by validated high performance thin layer chromatography and their antioxidant profile. J Pharm Biomed Anal (2014), 300-8.

  • Farrukh, M.R.; Ul A Nissar.; Sharma, P.R.; Tasduq, SA. Oxidative stress mediated calcium release manifests ER Stress leading to UPR in ultraviolet-B -irradiated human skin cells. Journal of Dermatological Sciences (2014), 24-35.

  • Lone, S.H.; Bhat, K.A.; Naseer, S.; Rather, R.A.; Khuroo, M.A.; Tasduq, SA. Isolation, cytotoxicity evaluation and HPLC-quantification of the  chemical constituents from Artemisia amygdalina Decne. J Chromatogr B Analyt Technol Biomed Life Sci (2013), 135-41.

  • Pathania, A.S.; Guru, S.K.; Verma, M.K.; Sharma, C.;Tasduq, SA.; Malik, F.; Chandra, S.; Katoch, M.; Bhushan, S. Disruption of the PI3K/AKT/mTOR signaling cascade and induction of apoptosis in HL-60 cells by an essential oil from Monarda citriodora. Food Chem Toxicol (2013), 246-254.

  • Khan, I.; Sangwan, P.L.; Dar, A.A.; Rafiq, RA.; Farrukh, M.R.; Dhar, J.K.;Tasduq, SA.; Koul, S. A validated high-performance thin-layer chromatography method for the identification and simultaneous quantification of six markers from Platanus orientalis and their cytotoxic profiles against skin cancer cell lines. J Sep Sci (2013), 2602-10.

  • Nissar, A.U.; Farrukh, M.R.; Kaiser, P.J.; Rafiq, R.A.; Afnan, Q.; Bhushan, S.; Adil, H.S.; Subhash, B.C.; Tasduq, SA. Effect of N-acetyl cysteine (NAC), an organosulfur compound from Allium plants, on experimentally induced hepatic prefibrogenic events in wistar rat. Phytomedicine (2013),  828-33.

  • Afnan, Q.; Adil, M.D.; Nissar-Ul, A.; Rafiq, A.R.; Amir, H.F.; Kaiser, P.; Gupta, V.K.; Vishwakarma, R.; Tasduq, SA. Glycyrrhizic acid (GA), a triterpenoid saponin glycoside alleviates ultraviolet-B irradiation-induced photoaging in human dermal fibroblasts.  Phytomedicine (2012), 658-64.

  • Farrukh, R.; Zargar, M.A.; Akhtar, A.; Tasduq, SA.; Surjeet, S.; Nissar, U.A.; Rakhshanda, S.; Masood, A.; Ganie, S.A.; Shajrul, A. Antibacterial and Antifungal Activity of Thymus serpyllum. Botany Research International (2012), 36-39.

  • Ali, F.; Sangwan,P.L.; Koul, S.; Pandey, A.; Bani, S.; Tasduq, SA.; Sharma, P.R.; Kitchlu, S.; Khan, I.A. 4-epi-Pimaric acid: a phytomolecule as a potent antibacterial and anti-biofilm agent for oral cavity pathogens. Eur J Clin Microbiol Infect Dis (2012), 149-59.

  • Yadav, Bhuvnesh.; Raina, Anupuma.; Tasduq, SA.; Dogra, T.D. Serological Polymorphisms of ABO and Rh(D) Blood Groups among Saraswat Brahmin Inhabiting Four States of North India. Anthropologist (2011), 17-20.

  • Imran, K.; Sangwan, PL.; Tasduq, S.A.; Gupta, B.D.; Dhar, J.K.; Manickavasagar, R.; Koul, S. Ten marker compounds-based comparative study of green tea and guava leaf by HPTLC densitometry methods: Antoxidant activity profiling. Journal of Separation Science (2011), 749-760.

  • Kamal, A.; Shetti, R.V.; Azeeza, S.; Swapna, P.; Khan, M.N.; Khan, I.A.; Sharma, S.; Tasduq, S.A. Anti-tubercular agents. Part 6: Synthesis and antimycobacterial activity of novel arylsulfonamido conjugated oxazolidinones. Eur J Med Chem (2011), 893-900.

  • Dar, A.M.; Peerzada, Kaiser.; Afzal, Z.M.; Tasduq, SA. Effect of Emblica officinalis (fruit) against UVB-induced photoaging in human skin fibroblasts. Journal of Ethnopharmacology (2010), 109-14.

  • Kamal, A.; Shetti, R.V.; Azeeza, S.; Ahmed, S.K.; Swapna, P.; Reddy, A.M.; Khan, I.A.; Sharma, S.; Tasduq, S.A. Anti-tubercular agents. Part 5: Synthesis and biological evaluation of benzothiadiazine 1,1-dioxide based congeners. Eur J Med Chem (2010), 4545-53.

  • Kaiser, P.; Youssouf, M.S.; Tasduq, S.A.; Singh, S.; Sharma, S.C.; Singh, G.D.; Gupta, V.K.; Gupta, B.D.; Johri, R.K. Medicinal role of a herbal product from Allium cepa (bulb) in the management of allergic reactions. Journal of Medicinal foods (2009), 374-382.

  • Sharma, S.; Khan, I.A.; Ali, I.; Ali, F.; Kumar, M.; Kumar, A.; Johri, R.K.;Tasduq, S.A.; Bani, S.; Pandey, A.; Suri, K.A.; Gupta, B.D.; Satti, N.K.;Dutt, P.; Qazi, G.N. Evaluation of Hydroxychavicol for its Antimicrobial, Antioxidant and Anti-inflammatory activity for its Potential Use as Oral Care Agent. Antimicrob Agents Chemother (2009), 216-222.

  • Tasduq, S.A.; Kaiser, P.J.; Gupta, B.D.; Gupta, V.K.; Johri, R.K. Negundoside; an irridiod glycoside from  leaves of Vitex negundo protects human liver cells against Calcium mediated toxicity –induced by Carbon tetrachloride. World J Gastroenterology (2008), 3693-709.

  • Mir, A.I.; Kumar, B.; Tasduq, S.A.; Gupta, D.K.;. Bhardwaj, S.; R.K. Johri. Reversal of hopatotoxin induced pre-fibrogenic events by Emblica officinalis-a histological study. Indian Journal of Experimental Biology, (2007), 626-9.

  • Tasduq, S.A.; Kaiser, P.; Sharma, S.C.; Johri, R.K. Potentiation of Isoniazid Induced Liver Toxicity by Rifampicin, In a Combinational Therapy of anti-tubercular Drugs (rifampicin, isoniazid and pyrazinamide) in Wistar Rats; A Toxicity Profile Study. Tasduq SA, Kaiser P, Sharma SC, Johri RK. Hepatology Research (2007), 845-853.

  • Sachin, B.S.; Sharma, S.C.; Sethi, S.;Tasduq, S.A.; Tikoo, M.K.; Tikoo, A.K.; Satti, N.K.; Gupta, B.D.; Suri, K.A,.;Johri, R.K.; Qazi, G.N. Herbal modulation of drug bioavailability: enhancement of rifampicin levels in plasma by herbal products and a flavonoid glycoside derived from Cuminum cyminum. Phytother Res. (2007), 157-63.

  • Johri, S.; Jamwal, U.; Kaiser, P.; Tasduq, S.A.; Rasool, S.; Verma, V.; Qazi, G.N. A novel peroxidase from Withania somnifera. Journal of Plant Sciences (2006), 72-79.

  • Tasduq, S.A.; Singh, K.; Satti, N.K.; Gupta, D.K.; Suri, K.A.; Johri, R.K. Terminalia chebula (fruit) prevents liver toxicity caused by sub-chronic administration of rifampicin, isoniazid and pyrazinamide in combination. Hum Exp Toxicol (2006), 111-8.

  • Tasduq, S.A.; Mondhe, D.M.; Gupta, D.K.; Baleshwar, M.; Johri, R.K. Reversal of fibrogenic events in liver by Emblica officinalis (Fruit), an Indian Natural Drug. Biological and Pharmaceutical Bulletin (2005), 1304 – 1306.

  • Tasduq, S.A.; Kaisar, P.; Gupta, D.K.; Kapahi, B.K.; Maheshwari, H.S.; Jyotsna, S.; Johri, R.K. Protective effect of a 50% hydro-alcoholic fruit extract of Emblica officinalis against anti-tuberculosis drugs induced liver toxicity. Phytotherapy Research (2005), 193-197.

  • Tasduq, S.A.; Peerzada, K.; Koul, S.; Bhat, R.; Johri, R.K. Biochemical manifestations of anti-tuberculosis drugs induced hepatotoxicity and the effect of silymarin. Hepatology Research (2005), 132-135.

  • Tasaduq, S.A.; Singh, K.; Sethi, S.; Sharma, S.C.; Bedi, K.L.; Singh, J.; Jaggi, B.S.; Johri, R.K.  Hepatocurative and Anti oxidant profile of HP-1, a poly herbal phytomedicine. Human and Experimental Toxicology (2003), 639-645.

Patents

  • Qazi, G.N.; Bedi, K.L.; Johri, R.K.; Tikoo, M.K.; Tikoo, A.K.; Sharma, S.C.; Tasduq, S.A.; Suri, O.P.; Gupta, B.D.; Suri, K.A.;. Satti, N.K.; Khajuria, R.K.; Singh, Surjit.; Khajuria, Anamika.; Kapahi, B.K. Bioavailability/bioefficacy enhancing activity of Zingber officinale, its extracts and fractions thereof. (Grant No. US20030170326 dt. 11/09/2003).

  • Johri, R.K.; Tasduq, S.A.; Singh, Kuldip.; Gupta, D.K.; Kapahi, B.K.; Mondhe, D.M.; Jain, S.M.; Suri, O.P.; Bedi, K.L.; Qazi, G.N. Herbal hapatocurative agent against CYP450 and free radical mediated drugs toxicity (including-tubercular drugs) (Dell No.NF53/2002. Grant No. US7001619 B2 dt. 21/02/2006).

  • Qazi, G.N.; Bedi, K.L.; Johri, R.K.; Tikoo, M.K.; Tikoo, A.K.; Sharma, S.C.; Tasduq, S.A.; Suri, O.P.; Gupta, B.D.; Suri, K.A.; Satti, N.K.; Khajuria, R.K.; Singh, Surjit.; Khajuria, Anamika.; Kapahi, B.K. Bioavailability/bioefficacy enhancing activity of Cuminum cyminum, its extracts and fractions thereof. (Dell No. 344 NF/2001, Grant No. US7514105 B2 dt. 07/04/2009).

  • Qazi, G.N.; Bedi, K.L.; Johri, R.K.; Sharma, S.C.; Tikoo, M.K.; Tikoo, A.K.; Tasduq, S.A.; Kuldeep, Singh.; Rashmi, Pandita.;  Suri, O.P.; Gupta, B.D.; Suri, K.A.; Satti, N.K.; Khajuria, R.K.; Kalsotra, A.K.; Neelam, Verma.; Rajneesh, Anand. New plant based agents as bioavailability/bioefficacy enhancers for drugs and pharmaceuticals. (Grant No. US 20040121028 A1 dt. 24/06/2004).

  • Qazi, G.N.; Bedi, K.L.; Johri, R.K.; Tikoo, M.K.; Tikoo, A.K.; Sharma, S.C.; Tasduq, S.A.; Suri, O.P.; Gupta, B.D.; Suri, K.A.; Satti, N.K.; Khajuria, R.K. Bioavailability enhancing activity of Carum carvi extracts and fractions thereof. (Grant no. WO2004067018 A1 dt 12/08/2004).

  • Koul, S.; Reddy, M.V.; Niranjan, T.; Koul, J.L.;Sangwan, P.L.; Taneja, S.C.; Khan, I.A.; Kumar, A.; Kalia, N.P.; Tasduq, S.A.; Johri, R.K.; Pagoach, S.S.; Tikoo, A.K.; Tikoo, M.K.; Saxena, A.K.; Agrawal, S.K.; Mondhe, D.M.; Agrawal, S.K.; Purnima, B.; Sharma, S.; Singh, S.; Singh, G.D.; Bhusari, S.; Qazi, G.N. Novel aromatic amides as potentiators of the bioefficacy of drugs (NF0113,13/JUN/08).

  • Koul, S.; Niranjan, T.; Reddy, M.V.; Sangwan, P.L.; Taneja, S.C.; Khan, I.A.; Kumar, A.; Fida, R.A.; Saxena, A.K.; Agrawal, S.K.; Johri, R.K.; Tasduq, S.A.; Singh, G.D.; Rao, BLN.; Dhar, A.K.; Purnima, B.; Qazi, G.N. Novel efflux pump inhibitors (NF 193(2007), 0525DEL2008).

PhD Students
  1. Nissar-Ul-Ashraf, Senior Research Fellow.

  2. Afnan Quadri, Senior Research Fellow

  3. Rafiq Ahmad Rather, Senior Research Fellow

  4. Love Sharma, Senior Research Fellow

  5. Nazir Ahmad Lone, Junior Research Fellow

Facilities

  • Cell culture facility

  • Biochemistry and Molecular Biology lab facilities

  • Photobiology Lab

  • Imaging Facilities (Fluorescence and Phase Contrast Microscopy)

  • Spectroscopy (Elisa reader, Fluorescene Spectrophotometery, Luminiscence spectrophotometry)

  • Gel documentation system