Dr. Sarojini Johri

Microbial Biotechnology Division .
CSIR - Indian Institute of Integrative Medicine,Canal Road, Jammu – 180001
Email: sjohri@iiim.ac.in , sarojini.johri@gmail.com

Positions Held  
Position Held Date Organization
Pr. Technical Officer   CSIR-IIIM
 
Membership  
  • Life Member, Indian Science  Congress Association (ISCA)

  • Life Member, Biotech Research Society of Indian  (BRSI)

  • Life Member, Society of Biological chemists of India (SBC)

  • Life Member, Punjab alumini Chandigarh

  • Member, Association of Microbiologists of India (AMI)

Research

Overview
Our main focus is on exploration and exploitation of India`s rich microbial diversity (both cultivable and uncultivable) for novel bioactive molecules and biocatalysts with application in health, agriculture and industry, and their overproduction using conventional and molecular biology tools. The group is also working on the expression of cryptic biosynthetic pathways of microorganisms.

Mission and goals

  • Development of microbial repository from extreme environments and endophytes of endemic plants

  • To study the plant- microbe interactions for disease management, adaption and optimum production of key metabolites in selected medicinal plants

  • Bioprospecting and characterization of novel endophytes and extremophiles for bioactives and biocatalysts

  • Development of metagenomic libraries from unexplored ecosystems for new/novel bioactives/biocatalysts

  • Production of microbial enzyme and high value microbial products from wild type and  genetically modified strains

  • Metabolic pathway engineering for production of novel/ desired metabolites

  • Hyperexpression and protein engineering of biocatalysts for their application in biotransformation of important drug auxillaries/intermediates.

Competencies

  • Repository of psychrotrophic, mesophilic and thermo-tolerant microorganism including bacteria, actinomycetes, fungi and endophytes.

  • Metagenomic libraries of environmental samples from unique niches.

  • High altitude medicinal plant gene bank .

  • Indigenous in vitro propagation methods for many high value medicinal plants.

  • Indigenous methods for rapid molecular detection of several microbial pathogens.

  • Indigenous novel expression system for heterologous protein production in fungi.

  • Hyperexpression and protein engineering .

  • Strain development for production of bioactive molecules and novel enzymes using traditional and molecular tools (genomics and proteomics).

Publications
  • Bhat, A.; Riyaz-Ul-Hassan, S.; Srivastava, N; Johri, S. Molecular cloning of rhodanese gene from soil metagenome of cold desert of North-West Himalayas: sequence and structural features of the rhodanese enzyme. (2014),DOI 10.1007/s13205-014-0249-2.

  • Bhat, A; Riyaz-Ul-Hassan, S; Ahmad, N; Srivastava, N; Johri, S. Isolation of cold-active, acidic endocellulase from Ladakh soil by functional metagenomics .Extremophiles,( 2013 ), 17:229-239.

  • Qadri, M.; Johri, S.; Shah, B A.; Khajuria , A .; Sidiq, T.; Lattoo , SK .; Abdin , MZ .;SR-Ul-Hassan. Identification and bioactive potential of endophytic fungi isolated from selected plants of the Western Himalayas SpringerPlus,( 2013), 2: -8.

  • Kumar, M.; Qadri, M.; Sharma, P. R.; Kumar, A.; Andotra, S.S. .; Kaur, T.; Kapoor, K.; Gupta, V. K. .; Kant, R.; Hamid, A.; Johri, Sarojini.; Subhash ,C. Taneja.; Vishwakarma, Ram A. .; Riyaz-Ul-Hassan, Syed.; Shah, Bhahwal Ali.  Tubulin Inhibitors from an Endophytic Fungus Isolated from Cedrus deodara. J. Nat. Prod , (2013),76, 194.

  • Syed, S.; Riyaz-Ul-Hassan, S.; Johri, S.     A novel exocellulase from an endophytic fungal strain DEF1. International journal of research in pharmaceutical and biomedical sciences,(2013), 4; 573-577.

  • Bhat, A.; Ahmad, N.; Srivastava, N.; Johri, S. Cloning and characterization of cellulase gene from soil metagenome Published in the Proceedings of the seminar on Bioresources and human sustenance (2013)held in Cotton College of Guwahati, Assam on 20-22 October 2011.

  • Saba,I.;Parvaiz, H..; Qazi , S.A.; Rather , R,A.; Dar , Q.A. Qadri , N. A.; Johri ,Sarojini Subash .;Taneja ,C. ;Shawl,Sami .2012    Purification and characterization of a cold active alkaline protease from Stenotrophomonas sp., isolated from Kashmir, India World J Microbiol Biotechnol. 28:1071-9.

  • Johri, S. ; Bhat, A. .; Sayed, S. ; Nargotra, A. ; Jain, A.; Qazi , G. N. .Novel thermostable lipase from Bacillus circulans IIIB153: comparison with the mesostable homologue at sequence and structure level. World J Microbiol Biotechnol,( 2012), 28:193–203.

  • Ahmad, N.; Johri, S.; Sultan, P.; Malik Z. A.; Qazi,G. N. Phylogenetic Characterization of Archaea in Saltpan . Indian J Microbiol.( 2011) ,51(2):132–137.

  • Ahmad, N.; Johri, S.; Abdin, M. Z.; Qazi, G.N. Molecular Characterization of Bacterial population in the forest soil of Kashmir, India. World J Microbiol Biotechnol,( 2009), 25: 107-113.

  • Riyaz-Ul-Hassan, S.; Syed, S.; Johri, S.; Verma, V.; Qazi, G.N. Application of a multiplex PCR assay for the detection of Shigella, Esch. coli and Shiga toxin-producing Esch. coli in milk. Journal of Dairy Research ,(2009),76; 188-194.

  • Ahmad, N.; sharma, S.; khan, F.G. .; kumar, R .;johri, S.; Abdin, M. Z. ; Qazi, G.N.  Phylogenetic analyses of archaeal ribosomal DNA sequences from saltpan sediment of Mumbai, India. Current Microbiology, (2008),57:145-152.

  • Maqbool, Q.; Johri, S.; Rasool, S.; Riyaz-ul-Hassan, S.; Verma, V. Nargotra, A.; Koul, S.; Qazi, G. N.  Molecular cloning of carboxylesterase gene & biochemical characterization of encoded protein from Bacillus subtilis (RRL BB1) J. Biotechnology. (2006),125, 1-10.

  • Johri, S. ; Jamwal, U. .; Peerzada, P. .; Tasduq, S. A. .;  Rasool, S. ; Verma, V. ; Qazi, G. N. A novel peroxidase from Withania somnifera. Journal of plant sciences.( 2006), 1, 72-79.

  • Kaiser, P.; Raina, C.; Parshad, R.; Johri, S.; Verma, V.; Andrabi, K. I. ; Qazi, G. N. A novel esterase from Bacillus subtilis (RRL 1789); Purification and characterization of the enzyme. Protein purification and expression,(2006), 45, 262-268.

  • Johri, S. ; Jamwal, U.; Rasool, S.; Verma, A.; Verma, V.; Qazi, G. N. Purification and characterization of peroxidases from Withania somnifera (AGB 002) and their ability to oxidize IAA. Plant Science, (2005 ),169, 1014–1021.

  • Rasool, S.; Johri, S.; Koul, S.; Subhash. C. ;Taneja,  S.; Maqbool.,Q.; Verma,V.; Qazi, G.N..Molecular cloning, nucleotide sequencing and properties of enantioselective ester hydrolase from Bacillus pumilus DBRL-191. FEMS Microbiol Letts, (2005), 249: 113-120.

  • Vakhlu, J.; Johri, S.; Verma, V.; Koul, S.; Parshad, R.; Taneja, S. C.; Qazi, G. N. Purification and properties of enantioselective ester hydrolase from a strain of Trichosporon species (DSMZ 11829). Enzyme Microbial Technology. (2005), 37: 330-339.

  • Qazi, P.H. ; Johri, S. ; Verma, V. ; Khan, L. ; Qazi, G.N. Cloning, sequencing and partial characterization of sorbitol transporter (srlT) gene encoding phosphotransferase system, glucitol/sorbitol-specific IIBC components of Erwinia herbicola ATCC 21998.Mol. Biol. Reports, (2004), 31: 143-149.

  • Maqbool, Q. A.;Johri, S.; Verma, L.; Riyaz-ul-Hassan, S.; Verma, V.; Koul, S.;    Taneja, S.C.; Parshad, R.; Qazi, G. N. Purification and characterization of a novel enantioselective hydrolase from Bacillus subtilis. Biotechnology & Applied  Biochemistry , (2002),  36(3): 227-234.

  • Johri, S.; Verma, L.; Verma, V.; Qazi, G.N. Phenol degradation by Pseudomonas species.  In Biotechnology in Agriculture and Environment , (2002), Ed. by JK. Arora, SS Marwaha and R. Grover, Asiatech Publishers Inc. New Delhi. P172-181.

  • Johri  ,S.; Verma, V.; Parshad, R.; Koul, S.; Taneja, S.C. ;Qazi, G.N. Purification and characterisation of an ester hydrolase from a strain of Arthrobacter species: Its application in asymmeterisation of 2-benzyl-1,3-propanediol acylates. Bioorganic & Medicinal Chemistry , (2001),  9, 269-273.

  • Vakhlu ,J.; Johri, S.; Verma, V.; Qazi, G.N.; Strategy based on plasmid incompatibility for Tn5 mutagenesis in Erwinia herbicola ATCC 21998.  Current Science, (1998), 74, 627-630.

  • Koul ,S.; Verma, V.; Johri, S .; Qazi, G.N. Physical characterization of a glucose dehydrogenase-bearing plasmid from ketacid producing Erwinia herbicola. World J. of Microbiol.& Biotecnol. (1995),  11, 234-235.

  • Johri, S.; Qazi ,G.N.; Chopra ,C.L. Evidence of plasmid dechlorinase activity in Pseudomonas sps. J. Biotechnol. (1991) ,20,73.

Team Members

1
Sarojini Johri
2
Syed Riyaz-Ul-Hassan
3
Rajinder Kumar
4
Jasbir Singh

Facilities

1
Well equipped Microbiology laboratories at IIIM, Jammu and branch laboratory Srinagar to carryout cutting edge research on isolation and characterization of microorganisms and their bioactive metabolites
2
State-of-the-art facility to undertake nucleic acid amplification, biomarker development, gene cloning, heterologous gene expression, protein purification, DNA sequencing and differential expression studies
3
Experimental farms at various locations in Jammu and Srinagar with a large collection of medicinal and aromatic plants suitable for studying plant-microbe interactions, endophytic, ectophytic and other symbiotic associations
4
Well equipped plant tissue culture Lab at Srinagar to carryout in vitro propagation studies for high value medicinal plants