Dr. Syed Riyaz-Ul-Hassan

Microbial Biotechnology Division
CSIR - Indian Institute of Integrative Medicine,Canal Road, Jammu – 180001
Email: srhassan@iiim.ac.in ; riyaz.andrabi@gmail.com

Positions Held  
Position Held Date Organization
Senior Scientist  2013 - Present CSIR-IIIM
Scientist 2009 - 2013 CSIR-IIIM
Scientist B 2006 - 2009 CSIR-IIIM
Honours & Awards  
  • Received BOYSCAST Fellowship from the Department of Science and Technology, New Delhi, to work with Prof. Gary Strobel at the Montana State University, USA for one year (21st July 2010-20th July 2011)

  • Qualified the Joint CSIR-UGC National Eligibility Test Dec. 2000.

  • Life member, Society of Biological Chemists, India, (No. 2991)

  • Life member, Association of Microbiologists of India (AMI/LM-376/2012)

  • Member, International Society for Fungal Conservation

Research Area

  • Plant-Microbe interactions in Crocus sativus L. (Saffron): I am undertaking research on Plant-microbe interactions in Crocus sativus L. In this project we are investigating the endophytic biodiversity, both bacterial and fungal, associated with the Saffron plant and explore them for plant growth promoting properties to understand the benefits that the plant is deriving from endophytism.  We are also trying to understand the molecular interactions between the endophytes and the host, and how these interactions affect the key metabolic pathways in the two symbiotic partners. Our aim is to establish useful plant-microbe associations in Saffron to improve the growth, adaptation and key metabolite content of the plant. Similar work is being carried out on some other medicinal/economically important plants.

  • Bioprospecting of endophytes for bioactive natural products: My research focuses on establishment of a repository of novel microorganisms, particularly endophytes for isolation and characterization of bioactive molecules including Volatile Organic Compounds. These organisms are obtained from extreme environments with plants bearing endemism, ethnobotanical applications and strong botanical biodiversity. Our rationale is based on the fact that certain microbes, selected from their natural ecological settings, may provide new and useful leads for industrial, medicinal and agricultural product discovery. The microbes are screened for antimicrobial, anti-cancer and immune suppressant activities. The endophytes are taxonomically characterized with conventional and molecular phylogeny. The bioactive compounds are purified and characterized. Potential bioactive microorganisms are selected for strain development to produce novel molecules.


Figure 1: Endophytic biology is studied with the aim of Bioprospecting for genuine microbial products, potential host metabolites and industrially important Volatile Organic Compounds (VOCs) or to understand the principles of endophytism and its consequences on the secondary metabolism of the partners as well as adaption of the plant host to biotic and abiotic stress conditions.

  • Development of molecular methods for the detection of foodborne pathogens: I am also undertaking work on Development of PCR and real-time PCR based assays for the detection of foodborne pathogens. We have developed real-time PCR assays for the detection of Salmonella, Shigella, Escherichia coli, Shigatoxin producing E. coli, Aspergillus flavus/A. parasiticus and Hepatitis B in our lab. The reactions were validated on the micro-PCR system fabricated by our collaborators. Further, protocols were devised for rapid and culture-independent detection of these pathogens in milk.

  • Wani, ZA.; Ashraf, N.; Mohiuddin, T.; Riyaz-Ul-Hassan ,S .Plant-endophyte symbiosis, an ecological perspective. Applied Microbiology and Biotechnology ,(2015) ,99(7): 2955-2965.

  • Qadri, M; Deshidi, R; Shah, BA; Bindu, K; Vishwakarma, RA; Riyaz-Ul-Hassan, S. An endophyte of Picrorhiza kurroa Royle ex. Benth, producing menthol, phenylethyl alcohol and 3-hydroxypropionic acid, and other volatile organic compounds. WorldJournal of Microbiology and Biotechnology (2015).  DOI: 10.1007/s11274-015-1910-6

  • Qadri ,M.;  Rajput, R.; Abdin, MZ.;  Vishwakarma ,RA.; Riyaz-Ul-Hassan, S . Diversity, molecular phylogeny and bioactive potential of fungal endophytes associated with the Himalayan blue pine (Pinus wallichiana). Microbial Ecology.,(2014), 67(4): 877-887. DOI: 10.1007/s00248-014-0379-4.

  • Nigg ,J.; Strobel, G.; Knighton, WB.; Hilmer ,J.; Geary, B.; Riyaz-Ul-Hassan,S.; Harper, JK.; Valenti, D.; Wang, Y. Functionalized para-substituted benzenes as 1,8-cineole production modulators in an endophytic Nodulisporium species. Microbiology.,(2014), 160: 1772-1782.

  • Bhat ,A.; Riyaz-Ul-Hassan ,S.; Srivastav,a N.; Johri, S. Molecular cloning of rhodanese gene from soil metagenome of cold desert of North-West Himalayas: sequence and structural features of the rhodanese enzyme. 3Biotech.,(2014), DOI 10.1007/s13205-014-0249-2.

  • Riyaz-Ul-Hassan ,S.; Verma, V .; Qazi ,GN. Real-time PCR based rapid and culture-independent detection of Salmonella in dairy milk- addressing some core issues. Letters in Applied Microbiology. ,(2013),56: 275–282 (Editor’s Choice paper of the month).

  • Zilla, MK.; Qadri ,M.; Pathania ,AS.; Strobel, GA.; Nalli ,Y.; Kumar, S.; Guru, SK.; Bhushan, S.; Singh ,SK.; Vishwakarma, RA.; Riyaz-Ul-Hassan, S.; Ali, A. Bioactive metabolites from an endophytic Cryptosporiopsis sp. inhabiting Clidemia hirta. Phytochemistry. , (2013),95: 291-297. doi:pii: S0031-9422(13)00249-5.

  • Kumar ,M.; Qadri ,M.; Sharma ,PR.; Kumar, A.; Andotra ,SS.; Kaur ,T.; Kapoor ,K.; Gupta, VK.; Kant, R.; Hamid, A.; Johri, S.; Taneja, SC.; Vishwakarma, RA.; Riyaz-Ul-Hassan, S.; Shah, BA . Tubulin Inhibitors from an Endophytic Fungus Isolated from Cedrus deodara. Journal of Natural Products. ,(2013),76(2): 194–199.

  • Riyaz-Ul-Hassan, S.; Strobel, G.; Geary, B.; Sears ,J .An endophytic Nodulisporium sp. from Central America producing volatile organic compounds with both biological and fuel potential. Journal of Microbiology and Biotechnology.,(2013), 23(1): 29–35.

  • Bhat ,A.; Riyaz-Ul-Hassan, S.; Ahmad, N.; Johri, S .Isolation of cold-active, acidic endocellulase from Ladakh soil by functional metagenomics. Extremophiles.,(2013) , 17(2): 229-239.

  • Qadri ,M.; Johri, S.; Shah ,BA.; Khajuria ,A.; Sidiq ,T.; Lattoo, SK.; Abdin ,MZ .; Riyaz-Ul-Hassan, S .Identification and bioactive potential of endophytic fungi isolated from selected plants of the Western Himalayas. SpringerPlus.,(2013) , 2: 8. doi:10.1186/2193-1801-2-8 (Highly accessed paper) .

  • Riyaz-Ul-Hassan ,S.; Strobel, G.; Booth ,E.; Knighton, B.; Floerchinger, C.; Sears, J. Modulation of volatile organic compound formation in the Mycodiesel® producing endophyte- Hypoxylon sp. CI-4. Microbiology,(2012), 158: 465–473.

  • Mends, MT.; Yu ,E.; Strobel ,GA.; Riyaz-Ul-Hassan ,S.; Booth, E.; Geary ,B .; Sears, J.; Taatjes ,CA.; Hadi, MZ. An endophytic Nodulisporium sp. producing volatile organic compounds having bioactivity and fuel potential. Journal of Petroleum and Environmental Biotechnology ,(2012) ,3:117. doi:10.4172/2157-7463.1000117

  • Kudalkar ,P.; Strobel ,G.; Riyaz-Ul-Hassan,S.; Geary ,B .; Sears J. Muscodor sutura, a novel endophytic fungus with volatile antibiotic activities. Mycoscience ,(2011),53 (4): 319-325.

  • Strobel ,G.; Singh, SK.; Riyaz-Ul-Hassan, S.; Mitchel ,AM.; Geary, B.; Sears J . An endophytic/pathogenic Phoma sp. from creosote bush producing biologically active volatile compounds having fuel potential. FEMS Microbiology Letters ,(2011),320: 87–94.

  • Ali ,SS.; Pervez ,R.; Andrabi ,R.; Sharma ,R .; Verma, V. Oscheius amsactae n. sp. (Nematoda: Rhabditida), a necromenic associate of red-hairy caterpillar, Amsacta moori (Lepidoptera: Arctiidae) from Kanpur district, India. Archives of Phytopathology and Plant Protection ,(2011),44: 871-878

  • Ali ,SS.; Andrabi, R.; Verma, V.; Ruchika.; Shaheen ,A.; Pervez, R.; Ali, S. Steinernema masoodi, S. seemae and other Indian isolates of Steinernema spp. Trends in Biosciences , (2010),3(2): 112-116

  • Riyaz-Ul-Hassan ,S.; Syed, S.; Johri ,S.; Verma ,V.; Qazi ,GN .Application of a multiplex PCR assay for the detection of Escherichia coli, Shigella and shiga toxin producing E. coli in milk. Journal of Dairy Research ,(2009) ,76: 188-194.

  • Rehman ,S.; Shawl, AS.; Sultana, S.; Kour ,A.; Riyaz-Ul-Hassan, S.; Qazi, GN. In vitro cytotoxicity of an endophytic fungus isolated from Nothophodytes foetida. Annals of Microbiology,(2009), 59: 157-161.

  • Riyaz-Ul-Hassan, S.; Verma, V.; Qazi, GN .Evaluation of three different molecular markers for the detection of Staphylococcus aureus by polymerase chain reaction. Food Microbiology ,(2008) ,25:452-459.

  • Rehman ,S.; Shawl ,AS.; Kour,A.; Riyaz-Ul-Hassan, S.; Sultan ,P.; Verma ,V.; Qazi, GN . An endophytic Neurospora sp.from Nothapodytes foetida producing camptothecin. Applied Biochemistry and Microbiology ,(2008),44:203-209.

  • Venugopal, SK.; Naik, S.; Somal ,P.; Sharma ,P.; Arjun, A.; Riyaz-Ul-Hassan .S.; Khajuria, RK .; Qazi ,GN . Production of 17-keto androstene steroids by the side chain cleavage of progesterone with Bacillus sphaericus. Biocatalysis and Biotransformation,(2007), 26: 272-279.

  • Maqbool, QA.; Johri ,S.; Rasool, S.; Riyaz-Ul-Hassan, S.; Verma, V.; Nargotra ,A.; Koul ,S.; Qazi ,GN. Molecular cloning of carboxylesterase gene and biochemical characterization of encoded protein from Bacillus subtilis (RRL-BB1).Journal of Biotechnology,(2006), 125: 1-10.

  • Puri ,SC.; Nazir, A.; Chawla ,R.; Arora, R.; Riyaz-Ul-Hassan, S.; Amna ,T.; Ahmad, B.; Verma, V.; Singh, S.; Sagar ,R.; Sharma, A.; Kumar, R.; Sharma, RK.; Qazi ,GN. The endophytic fungus Trametes hirsuta as a novel alternative source of podophyllotoxin and related aryl tetralin lignans. Journal of Biotechnology , (2006), 122 (4): 494- 510.

  • Rasool ,S.; Johri ,S.; Riyaz-Ul-Hassan, S.; Verma,V.; Koul, S.; Taneja ,SC.; Qazi ,GN. Molecular Cloning, nucleotide sequencing, and properties of an enantioselective ester hydrolase from Bacillus pumilus DBRL191. FEMS Microbiology Letters , (2005), 249: 113-120.

  • Ahmad ,K.; Koul ,S.; Taneja, SC.; Singh, AP.; Kapoor, M.; Riyaz-Ul-Hassan ,S.; Verma ,V .; Qazi ,GN. Enzyme directed diastereoselectivity in chemical reductions: studies towards the preparation of all four isomers of 1-phenyl-1,3-butanediol. Tetrahedron: Asymmetry ,(2004), 15: 1685–1692.

  • Riyaz-Ul-Hassan ,S.; Verma, V.; Qazi, GN . Rapid detection of Salmonella by polymerase chain reaction. Molecular and Cellular Probes ,(2004),18: 333-339.

  • Riyaz-Ul-Hassan ,S.; Verma, V.; Malik ,A.; Qazi ,GN .Microbiological characteristics of walnut kernels and apple juice concentrate. World Journal of Microbiology and Biotechnology,(2003), 19: 845-850.

  • Maqbool ,QA.; Johr,i S.; Verma, L.; Riyaz-Ul-Hassan, S.; Verma, V.; Koul ,S.; Taneja, SC.; Parshad, R.; Qazi ,GN. A novel enantioselective hydrolase from Bacillus subtilis; Purification and characterization. Biotechnology and Applied Biochemistry ,(2002),36: 227-234.


  • Qazi, GN.; Verm, V.; Riyaz-Ul-Hassan ,S. Oligonucleotide primers of sequenceID. Nos. 1-21 and a process for detection of Salmonella using oligonucleotide primers. (US10/440, 211. No. 7,041,482, May 9, 2006).

  • Qazi, GN.; Verma ,V.; Riyaz-Ul-Hassan ,S. Oligonucleotide primers of sequence ID. Nos. 1-21 and a process for detection of Salmonella using oligonucleotide primers.( IN 225146, dt. Dt. 30/09/2004, Grated 03/11/2008).

  • Qazi ,GN.; Puri, SC.; Braroo, A.; Verma, V.; Riyaz-Ul-Hassan, S.; Anand, R.; Bhardwaj, LM.; Bhondakar ,AP.; Kumar ,A.; Bajpai, RP. Microbial decontaminator. (WO/2004/087221. Oct. 14 2004).

CSIR Fellow

Masroor Qadri, SRF
Zahoor Ahmad Wani, SRF
Dania Nazir Mirza, JRF
Palak Arora, JRF