Dr. Prashant Misra

Plant Biotechnology Division
CSIR - Indian Institute of Integrative Medicine,Canal Road, Jammu – 180001
Email: pmisra@iiim.ac.in

Positions Held  
Position Held Date Organization
Scientist 2012 - Present CSIR-IIIM
Honours & Awards  
  • Qualified UGC-CSIR JRF and NET Exam.

  • Awarded CSIR-SRF.

  • Awarded Alexander von Humboldt Fellowship for post-doc Research at Technical University of Munich, Germany.

  • Recognized as Ph.D. examiner of Banaras Hindu University, Varanasi, India.

  • Reviewing for prestigious international journals such as PLOS ONE, SCIENTIFIC REPORTS (NPG), FUNCTIONAL AND INTEGRATIVE GENOMICS, BMC GENOMICS, JOURNAL OF PLANT GROWTH REGULATION etc.
Membership  
  • Life Member of The society of Biological Chemists, Bangalore, India

Research Area

Plant Molecular Biology
Tools and techniques: Genetic engineering, Transgenic plants, Transcriptome sequencing

Activities:

(A) Investigation of transcriptional regulation of plant secondary metabolism
One of the research activities of my research group is to understand transcriptional regulation of genes involved in biosynthesis of valuable secondary metabolites such as flavonoids and terpenoids. The overall aim is to identify key transcription factor genes and to use them in metabolic engineering of targeted group of secondary metabolites. In this context, we are studying regulatory role of R2R3 MYB family transcription factors in model plant Nicotiana tabacum.

Figure showing glandular trichomes over aerial plant parts of tobacco

(B) Glandular trichomes: Metabolism, gene expression and development
Glandular trichomes are known for highly active machinery to biosynthesize an array of valuable secondary metabolites. Currently, there is very limited knowledge pertaining to mechanism of gene regulation in these structures. Using tobacco as a model system, we are studying transcriptional regulation of structural genes involved in glandular trichome specific secondary metabolism. Another related research activity is to study role of homedomain zipper (HDZIP) family of transcription factors in regulation of epidermal cell differentiation and trichome development.

(B) Exploring plants as expression hosts for therapeutic proteins
Plants are alternate expression system for therapeutic proteins. The recent FDA approval of a therapeutic protein, produced from genetically engineered plant cell has ignited renewed interest in this area. With a long term aim to explore plants as expression hosts for therapeutic proteins, we have initiated research activities focusing on this area.

Publications

  • Pandey, A.; Misra, P.; Choudhary, D.; Yadav, R.; Goel, R.; Bhambhani, S.; Sanyal, I.; Trivedi, R.; Trivedi, P.K. AtMYB12 expression in tomato leads to large scale differential modulation in transcriptome and flavonoid content in leaf and fruit tissues. Scientific Reports (Nature Publishing group) (*Shares equal first authorship) Manuscript accepted for publication.(2015)

  • Pandey, A.; Misra, P.; Trivedi, P.K. Constitutive expression of Arabidopsis MYB transcription factor, AtMYB11, in tobacco modulates flavonoid biosynthesis in favour of flavonol accumulation. Plant Cell Reports (2015) DOI 10.1007/s00299-015-1803-z

  • Pandey, A.; Misra, P.; Bhambhani, S.; Trivedi, P.K. Expression of Arabidopsis MYB transcription factor, AtMYB111, in tobacco also requires light to modulate flavonol content. Scientific Reports (2014) (Nature Publishing Group)

  • Pandey, A.; Misra, P.; Khan, M.P.; Swarnkar, G.; Tewari, M.C.; Bhambhani, S.; Trivedi, R.; Chattopadhyay, N.; Trivedi, P.K. Co-expression of Arabidopsis transcription factor, AtMYB12, and soybean isoflavone synthase, GmIFS1, genes in tobacco leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. Plant Biotechnology Journal (2013) 12(1):69-80.

  • Pandey, A.; Misra, P.; Chandrashekhar, K.; Trivedi, P.K. Development and optimization of AtMYB12 expressing transgenic tobacco callus culture for large scale production of rutin with biopesticidal potential. Plant Cell Reports (2012) 31:1867-1876.

  • Misra, P.; Pandey, A.; Tewari, S.K.; Nath, P.; Trivedi, P.K. Characterization of isoflavone synthase gene of Psoralea corylifolia, a medicinal plant. Plant Cell Reports (2010) ,29, 747-755.

  • Misra, P.; Pandey, A.; Tewari, M.; Chandrashekha,r K.; Siddhu, O.P.; Asif, M.H.; Chakrabarty, D.;   Singh, P.K.; Nath, P.; Trivedi, P.K.; Tuli, R. Modulation of transcriptome and metabolome by AtMYB12 transcription factor leads to insect tolerance. Plant Physiology (2010) 152, 2258-2268.

  • Rai, A.; Bhardwaj, A.; Misra, P.; Bag, S.; Adhikari, B.; Trivedi, P.; Tripathi, R; Chakrabarty, D. Comparative transcriptome analysis of contrasting rice genotypes for accumulation and tolerance to arsenic. The Plant Genome (2015), 8: 1-14.

  • Dubey, S.; Shri, M.;  Misra, P.; Lakhwani, D.; Bag, S.; Asif, M.H.; Trivedi, P.K.; Tripathi, R.D.; Chakrabarty, D. Heavy metals induce oxidative stress and genome wide modulation in transcriptome of rice roots. Functional and Integrative Genomics,(2014) 14(2):401-17.

  • Dubey, S.; Misra, P.; Dwivedi, S.; Chatterjee, S.; Bag, S.; Mantri, S.; Asif, M.H.; Rai, A.; Kumar, S.; Shri, M.; Tripathi, P.; Tripathi, R.D.; Trivedi, P.K.; Chakrabarty, D.; Tuli, R. Transcriptomic and metabolomic shifts in rice roots in response to Cr (VI) stress. BMC Genomics (2010) 11, 648.

  • Shri, M.; Rai, A.; Verma, P.K.; Misra, P.; Dubey, S.; Kumar, S.; Verma, S.; Gautam, N.; Tripathi, R.D.; Trivedi, P.K.; Chakrabarty, D. An improved    Agrobacterium-mediated transformation of recalcitrant indica rice (Oryza sativa L.) cultivars. Protoplasma (2013),250:631-636.

  • Pathak, S.; Mishra, B.K.; Misra P.; Misra, P.; Joshi, V.K.; Shukla, S.; Trivedi, P.K. High frequency somatic embryogenesis, regeneration and correlation of alkaloid biosynthesis with gene expression in Papaver somniferum. Plant Growth Regulation (2012) ,68:17-25.

  • Chakrabarty, D.; Trivedi, P.K.; Misra, P.; Tiwari, M.; Shri, M.; Shukla, D.; Kumar, S.; Rai, A.; Pandey, A.; Nigam, D.; Tripathi, R.D. and  Tuli, R.Comparative transcriptome analysis of arsenate and arsenite stresses in rice seedlings. Chemosphere (2009),74, 688–702.

  • Chakrabarty, D.; Trivedi, P.K.;  Shri, M.;  Misra, P.; Asif,  M.H.; Dubey, S.; Kumar, S.; Rai, A.;  Tiwari, M.; Shukla, D.;  Pandey, A.; Nigam, D. and Tuli,  R. Differential transcriptional expression following thidiazuron induced shoot primordia developmental shift in rice. Plant Biology (2009),12, 46-59.

  • Shri, M.; Kumar, S.; Chakrabarty, D.; Trivedi, P.K.; Mallick, S.; Misra, P.; Shukla, D.; Mishra, S.; Srivastava, S.; Tripathi, R.D. and Tuli, R. Effect of arsenic on growth, oxidative stress and antioxidant system in rice seedlings. Ecotoxicology and Environmental Safety (2009), 72, 1102-1110.

  • Asif, M.H.; Trivedi, P.K.; Misra, P.; Nath, P. Prolyl-4-hydroxylase (AtP4H1) mediates and mimics low oxygen response in Arabidopsis thaliana. Functional and Integrative Genomics (2009), 9, 525-535

Current Students

AcSIR Students
1
Meenakshi Sharma (JRF)
2
Umar Gani (JRF)
Project Fellow
3
Abhishek Nautiyal