Dr. Meenu Katoch

Microbial Biotechnology Division
CSIR - Indian Institute of Integrative Medicine,Canal Road, Jammu – 180001
Email: mkatoch@iiim.ac.in

Positions Held  
Position Held Date Organization
Visiting Fellow 2014-2015 UNSW, Aus
Senior Scientist 2011-Cont CSIR-IIIM
Scientist 2007-2011 CSIR-RRL
Jr. Scientist 2004-2007 CSIR-RRL
 
Honors and Awards  
  • Recieved the DBT Travel support for FEMS Congress of European microbiologist held at Maastricht, The Netherlands 6201.
  • Recieved the DBT-CREST fellowship for 2012-2013 (Formerly  Biotechnology Overseas Associateship-BTOA).
  • Scrutinized for the Human Resource Development Scheme of Department of Health Research for Fellowship/ Training in foreign Institutes (2012-2013).
  • Qualified National Eligibility Test for Lectureship 1998, 1999 conducted by Indian Council of Agricultural Research.
  • DBT Scholarship for M.Sc. Biotechnology.
  • Senior Research Fellowship from DBT for Ph.D.
  • Borkataki S, Katoch R, Yadav A, Khajuria JK, Katoch M, Goswami P, Kaur R, Incidence of parasitic infection in cats of Jammu. Presented in 20th National Congress on ‘Parasitology Today – Ecology to Molecular Biology’ from Feb 18-20th, 2010 held at COVSc, HAU, Hisar- 125004 Best poster Award.
  • Yadav A, Katoch M, Katoch R, Khajuria J.K, Vohra S, Sood S. Prevalence of cryptosporidiosis in diarrhoeic buffalo and cow calves in and around Jammu. Presented in National Congress on ‘National Impact of Parasitic Diseases on Livestock Health and Production’ from Feb 3-5th, 2009 held at GADVASU, Ludhiana- 181102 Best poster Award.
  • Katoch M, Yadav A, Qazi G. N. (2007) Cryptosporidium infection in diarrhoeic calves and human in and around Jammu Presented in symposium on ‘Emerging Parasitic Zoonoses and Advances in Herbal Medicine against Parasites of Veterinary Importance’ from Sep 7-9th, 2007 held at FVSc & AH, R.S.Pura, SKUAST-J, 181102 Best paper Award in “Technical Session V-Zoonoses” .
  • Yadav A, Katoch R, Vohra S., Khajuria J.K., Katoch M, Sood S, Agrawal R, Gupta A, Srivastava A.K. (2007). Parasitic Infection of wild animals and birds in and around Jammu. Presented in International Symposium on "Advances in zoo and wild animals health and management” from April 26-27, 2007 at FVSc & AH, R.S.Pura, SKUAST-J, 181102 Best poster Award in “Disease of Wild & Zoo animals and their treatment” session.

  • Katoch R, Yadav A, Panda A.K., Agnihotri R.K. and Katoch M. (2006). Prevalence of Gastrointestinal Parasites in Dogs of North Western Himalayan Region. Presented in International Symposium on "New Strategies for Prevention and Control of Emerging and Re-emerging Zoonoses – An Integrated Veterinary and Medical Approach” from 12th to 14th October, 2006 at COVAS, HPKV, Palampur, H.P. pp 155 Second Best Poster Awardin “Animal Health and Disease control strategies” session.

Membership  
  • Life member of Indian Society of Virology
  • Association of Microbiologists of India 
 

Research Area

RD Projects (pursued)

  • PCR based diagnosis of Cryptosporidium spp, sponsored by DST, Govt. of India (Project Leader, 2004-2007)

Filamentous fungi have ability to produce proteins in large quantity (30g/l). To exploit this quality of filamentous fungi and non-availability of commercial expression system, an expression system was developed. Two promoters (of glucose oxidase/catalase gene) from Aspergillus niger strain were isolated and used in the development of expression vector for expressing the heterologous proteins. Under these promoters, expression of reporter proteins was upregulated suggesting the efficient working of system.

  • Novel Expression System for filamentous fungus sponsored by CSIR under NMITLI, (Principle Investigator, 2005-2008)

Filamentous fungi have ability to produce proteins in large quantity (30g/l). To exploit this quality of filamentous fungi and non-availability of commercial expression system, an expression system was developed. Two promoters (of glucose oxidase/catalase gene) from Aspergillus niger strain were isolated and used in the development of expression vector for expressing the heterologous proteins. Under these promoters, expression of reporter proteins was upregulated suggesting the efficient working of system.

  • Bioprospection and development of technologies for the production of bioactive molecules from the microbial wealth of north western region, sponsored by CSIR (Lab project), Govt of India (Principle Investigator, 2009-2012)
Current RD Projects
  • Bioprospection and development of technologies for the production of bioactive molecules from the microbial wealth of north western region, sponsored by CSIR (Lab project), Govt of India

Our natural product discovery program is focused upon the isolation and structure elucidation of novel and/or bioactive molecules from microorganisms with the aim of discovering new chemical entities as well as understanding their biosynthesis. Microorganisms are a proven source of medically relevant natural products, many of which belong to the polyketide (e.g erythromycin) and non-ribosomal peptide (e.g penicillin) structure classes. The presence of biosynthesis genes encoding the assembly of non-ribosomal peptides, polyketides and some alkaloids in microorganisms is indicative of a potentially rich source of bioactive and novel natural products. Our library of cultured microbes is derived from numerous sources, predominantly plants, and most of these microorganisms have never been investigated for the sole purpose of small molecule discovery. The initial phase of our multifaceted screening process incorporates elements of molecular and microbiology together with biological assays. These approaches when coupled with chemical screening form the basis of our dereplication process allowing us prioritise microbes for further investigation.

  • Exploration of micro-flora isolated from north western Himalayas for anticancer molecules DBT SPONSORED PROJECT

The project seeks to establish a repository of endophytes and isolation and characterization of novel bioactive agents. These organisms are obtained from specific areas of the state in which there are areas of endemism, ethnobotanical applications of plants and strong botanical biodiversity. Our rationale is based on the fact that certain microbes, selected from their natural plant ecological settings, may provide new and useful leads in industrial, medicinal and agricultural product discovery. The microbes are screened for anti-cancer activities. The endophytes are taxonomically characterized with conventional and molecular phylogeny. Preference is given to microbes representing unique texa. The bioactive compounds are purified and characterized. The project will lead to establishment of a repository of endophytic microorganisms, isolation of new bioactive molecules against cancer. Potent bioactive microorganisms will be selected for metabolic engineering of the polyketide pathway to produce new and desired molecules. It will also generate intellectual property and publications

  • Exploration of myxobacteria from the North Western Himalayas for novel enzymes and secondary metabolites ICAR SPONSORED PROJECT

Many pathogens are able to generate resistances against drugs like antibiotics leading to an uncontrolled spreading of pathogenic strains. However, an efficient treatment of numerous diseases is missing due to the lack of specific drugs. Therefore, a major task in pharmaceutical research is the identification and development of new agents. Interesting targets are natural products, so-called secondary metabolites, synthesized by microbes, plants and fungi. These agents can, for example, act as antibiotics, anti-cancer drugs, cholesterol-lowering agents, immunosuppressants, anti-parasitics and anti-diabetics.
            One of the large-scale producers of natural products are myxobacteria, which are living in soil and secrete a number of compounds not only to kill their microbial competitors or enemies. This bacterium has a huge capacity to produce natural products. The data allow the optimization of metabolite production in these microbes and the expression of complete pathways in heterologous hosts. Furthermore, it is possible to perform predictable alterations to the agents’ structures by genetic engineering. Experiments are underway to “mine” the sequenced genomes for novel compounds using state-of-the-art analytical techniques as well as to deepen the understanding of host microbiology.
Our lab also run a myxobacterial strain-discovery program, which results in the identification of new myxobacterial species, genera and families. Once a new strain has been successfully adapted to growth under laboratory conditions, we use state-of-the-art mass spectrometric techniques to screen the strain's metabolite profile with respect to the presence of known myxobacterial secondary metabolites. At the same time we perform a range of biological assays, using indicator organisms as well as cell-based screening approaches to discover novel compounds exhibiting a potentially interesting activity. Following optimization of the production of candidate compounds, cultivation of the producing strain is upscaled and the target compounds are purified using liquid chromatography methods. Thereafter, structure elucidation using a panel of analytical techniques including multidimensional NMR spectroscopy is carried out. In addition, further cell-based experiments are performed in order to investigate in detail the mode-of-action of a novel natural product.
Scientists in the interdisciplinary research group combine a broad spectrum of techniques including microbiological, molecular-biological, genetic, biochemical, analytical and bioengineering methods.

  • Heterologous expression of biosynthetic gene cluster of small molecules

The MAA biosynthetic gene cluster of Cylindrospermum stagnale was cloned in pET28 vector and was expressed in E.coli BL21 (DE3) pRARE strain. Extracts were prepared under induced and un-induced conditions and run on HPLC. Two extra peaks were observed in induced condition. Mass spectrometry and NMR data of purified peaks, suggested, that this biosynthetic gene cluster is producing mycosporine ornithine and mycosporine lysine.

HPLC

  Mycosporine ornithine Mycosporine lysine
Mass 303 317
MS2 235, 267, 285 217, 281, 298
MS3

235:  191
267:  235

285:   NF

217: NF
281: NF

298: NF
  • Plant microbial and Soil Interaction project CSIR NETWORKING PROJECT

Understanding the molecular basis of plant microbe and soil interactions  Atropa acuminate.

Publications
  • Godara, R.;  Katoch, R.;  Yadav, A.;  Ahanger, R.R.; Bhutyal, A.D.S.; Verma, P.K.; Katoch, M.; Dutta, S.; Nisa, F.; Singh, N.K. In vitro acaricidal activity of ethanolic and aqueous floral extracts of Calendula officinalis against synthetic pyrethroid resistant Rhipicephalus (Boophilus) microplus. Experimental and Applied Acarology (2015), DOI:10.1007/s10493-015-9929-9.                                                                     

  • Katoch, M.; Khajuria, A. Sharma, P.R.; Saxena, A.K. Immunosuppressive potential of Botryospharia dothidea, an endophyte isolated from Kigelia africana. Pharmaceutical Biology (2015), 85-91                                                                                      

  • Godara, R.;  Parveen, S.; Katoch, R.;  Yadav, A.;  Katoch, M.; Khajuria, J.K.; Kaur, D.; Ganai, A.;  Verma, P.K.; Khajuria, V.; Singh, N.K. Acaricidal activity of ethanolic extract of Artemisia absinthium against Hyalomma anatolicum ticks. Experimental and Applied Acarology (2015), 141-148.                                                                                       

  • Parveen, S.;  Godara, R.;  Katoch, R.;  Yadav, A.;  Verma, P.K.; Katoch, M.; Singh, N.K. In vitro evaluation of ethanolic extracts of Ageratum conyzoides and Artemisia absinthium against cattle tick Rhipicephalus microplus. The Scientific World Journal (2014), 858973, 1-6                                                                                          

  • Godara, R.; Katoch, M.; Katoch, R.; Yadav, A.; Parveen, S.; Vij, B.; Khajuria, V.; Singh, G.; Singh, N.K. In vitro Acaricidal Activity of Atropa belladonna and its Components, Scopolamine and Atropine against Rhipicephalus microplus. The Scientific World Journal (2014), 713170: 1-6

  • Katoch, M.; Salgotra, A.; Singh, G. Endophytic Fungi Found In Association With Bacopa monnieri As Resourceful Producers Of Industrial Enzymes And Antimicrobial Bioactive Natural Products. Brazilian Archives of Biology and Technology (2014), 714-722.                 

  • Katoch, M.; Singh, G.; Sharma, S.; Gupta, N.; Sangwan, P.L.; Saxena, A.K. Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell (Scrophulariaceae) BMC Complementary and Alternative Medicine (2014), 52, 1-8.                       

  • Yadav, A.; Katoch, R.; Godara, R.; Sood, S.; Katoch, M.; Ahamed, I. Field efficacy of minidosed pour-on ivermectin and eprinomectin against goat warble fly infestation by Przhevalskiana silenus. Tropical Animal Health and Production (2014), 537-539.                

  • Godara, R.; Parveen, S.; Katoch, R.; Yadav, A.; Verma, P.K.; Katoch, M.; Kaur, D.; Ganai, A.; Raghuvanshi, P.; Singh, N.K. Acaricidal activity of extract of Artemisia absinthium against Rhipicephalus sanguineus of dogs. Parasitology Research (2014), 747-754.                  

  • Pathania, A.S.; Guru, S.K.; Verma, M.K.; Sharma, C.; Abdullah, S.T.; Malik, F.; Chandra, S.; Katoch, M.; Bhushan, S. Disruption of the PI3K/AKT/mTOR signaling casxade and induction of apoptosis in HL-60 cells by an essential oil from Monarda citriodora. Food and Chemical Toxicology (2013), 246-254.                                                                

  • Katoch, M.; Hussain, M.A.; Ahuja, A. Comparison of SSR and cytochrome P-450 markers for estimating genetic diversity in Picrorhiza kurrooa L. Plant Systematics and Evolution(2013), 1637-1643.    

  • Katoch, M.; Bhushan, S.; Katoch, A.; Kumar, R.; Kitchlu S. Streptomyces spiroverticillatus from the rhizospheric soil of Arnebia euchroma: its antimicrobial and anticancer potential. International Journal of Advances in Pharmaceutical Research (2013), 1603-1607.

  • Sharma, R.; Katoch, M.; Govindappa, N.; Srivastava, P.S.; Shastry, K.; Qazi G. N. Evaluation of Catalase promoter for expressing alkaline xylanase gene in the Aspergillus niger. FEMS Microbiology Letters (2012), 33-40.     

  • Yadav, A.; Katoch, R.; Khajuria, J.K.; Katoch, M.; Rastogi, A. Economic impact of Prezhelvalskiana silenus infestation in native goats of north India. Tropical Animal Health and Production (2012), 581-587. 

  • Yadav, A.; Katoch, R.; Khajuria, J.K.; Katoch, M.; Agarwal, R. Prevalence and biology of goat warble fly infestation by Prezhelvalskiana silenus in Jammu province, India. Tropical Animal Health and Production (2011), 1487-1492.       

  • Katoch, M.; Fazli, I.S.; Suri, K.A.; Qazi, G.N. Effect of altitude on picroside content in core collections of Picrorhiza kurrooa from north western Himalayas. Journal of Natural  Medicine (2011), 578-582.  

  • Katoch, M.; Kumar, R; Pal, S; Ahuja, A Identification of Chlorophytum species (C. borivilianum, C. arundinaceum, C. laxum, C. capense and C. comosum) using molecular marker. Industrial Crop and Products (2010), 389-393.

  • Ahlawat, A.; Katoch, M.; Gandhi, R.; Ahuja, A. Genetic diversity in Acorus calamus L. as revealed by RAPD markers and its relationship with β-asarone content and ploidy level. Scientia Horticulturae(2010), 294-297.     

  • Sharma, R.; Katoch, M.; Srivastava, P.S.; Qazi, G.N. Approaches for refining heterologous protein production in filamentous fungi. World Journal of Microbiology and Biotechnology (2009), 2083-2094. 

  • Katoch, M.; Abdin, M.Z.; Zaidi A.A. First report of Tobacco rattle virus occurring in Gladiolus in India. Plant Pathology (2004), 236.

  • Katoch, M.; Raja Ram; Zaidi, A.A. First report of Tobacco ringspot virus occurring in Gladiolus in India. Plant Pathology (2003), 789.  

  • Katoch, M.; Abdin, M.Z.; Raja Ram; Zaidi, A.A. An Overview of Diagnostics For Viruses Infecting Gladiolus. Crop protection (2002), 153-156.

  • Katoch, M.; Raja Ram; Zaidi A.A.; Garg, I.D. Status of Bean Yellow Mosaic Virus on Gladiolus. Crop Protection (2002), 861-865.

  • Katoch, M.; Kataria, J.M.; Katoch, V.C. Immunogenicity of Fowl pox virus polypeptides. International Journal of Animal Sciences (1999), 269-271.

  • Garg, M.; Kataria, J.M. Humoral and cellular immune response to   virulent Fowl pox virus (I.V.R.I. Mukteshwar Strain) Indian Veterinary Journal (1996),127-130.

  • Sharma, K.D.; Singh, B.M.; Sharma, T.R.; Katoch, M.; Guleria, S. Molecular analysis of variability in Podophyllum hexandrum Royle-an endangered medicinal herb of northwestern Himalaya. Plant Genetic Resources Newsletter (2000) 124, 57-61.   

  • Katoch, V.C.; Ram. G.C.; Bansal, M.P.; Meenu Effect of stimulation with LPS on immune phagocytosis by sheep peripheral blood mononuclear cells. Indian Veterinary Journal (2000), 95-97.

  • Katoch, V.C.; Ram. G.C.; Bansal, M.P.; Meenu ANE staining pattern of ovine peripheral blood monocyte during in-vitro culture. Indian Veterinary Journal (1999), 282-284.

  • Katoch, V.C.; Ram. G.C.; Bansal, M.P.; Meenu Quantitation of Acid phosphatase  activity of ovine mononuclear phagocyte in-vitro. Indian Veterinary Journal (1998), 419-422.

  • Katoch, V.C.; Ram. G.C.; Bansal, M.P.; Meenu Intrinsic antiviral activity of sheep peripheral blood mononuclear phagocyte against vero-adapted sheep pox virus strain. International Journal of Animal Sciences (1998), 113-114.

Patents:

Granted Patent

  • Singh, B.; Katoch, M.; Ram, R.; Zaidi,  A.A. A new antiviral agent from Indian   horse  chestnut aesculus indica.  (Grant No. EP 1489910  dt.11//04/2007)

  • Singh, B.; Katoch, M.; Ram, R.; Zaidi,  A.A. A new antiviral agent from Indian   horse  chestnut aesculus indica.  (Grant No. 4363989 dt 28/08/2009)

  • Singh, B.; Katoch, M.; Ram, R.; Zaidi,  A.A. A new antiviral agent from Indian   horse  chestnut aesculus indica.   (Grant No. WO/2003/079795 PCT/IB2002/001129 dt 02/10/2003) 

Filed Patents

  • Katoch, M.; Zaidi, A.A.; Ram, R. Development of diagnostic kit for the detection of Bean Yellow Mosaic Virus. (File No. 76/NF/2002) 

  • Katoch, M.; Zaidi, A.A.; Ram, R. A process for developing Diagnostic Kit for detection of Bean Yellow Mosaic Virus in commercial crop (File No. 17/NF/2002)  

  • Katoch, M.; Sharma R.; Qazi G.N. Promoter Sequence of Catalase R gene of A. niger and a method for expressing foreign genes in Aspergillus. (Del No. 1006DEL2010 dt 27/04/2010)

  • Katoch, M.; Qazi G.N. Glucose oxidase promoter for use in Aspergillus. (Del No. 2125DEL2009 dt 13/10/2009)

Book:

  • Sharma, R; Katoch, Meenu; Srivastava, PS. Aspergillus niger as expression host for protein production. Lambert aced Publishing, Germany.(2013) ISBN-13 978-3-659-32138-2

Chapter in Book:

  • Katoch, M; Singh, G; Vishwakarma. Molecular Mechanism of Cellulase Production Systems in Trichoderma. In: Trichoderma: Their uses and Applicationsin Bioengineering and Biotechnology (eds) Gupta VK, Schmall M, Herrera-Estrella A., Druzhinina I., Upadhyay RS., Tuohy M. M. 22: pp. 319-324. Elsevier Publication,(2013), ISBN: 978-0-444-59576-8 .

  • Yadav ,A.; Katoch, M. Cryptosporidiosis in bovine and human In: Veterinary Parasitology (eds) Katoch R, Godara R, Yadav A 4: pp Published by Satish Serial Publishing House (2013), ISBN 9789381226896.

Current Students

1
Mr. Gurpreet Singh
2
Ms. Arushi Sharma
3
Ms. Rubby Sandhu

Alumini

Ruchika Sharma-“Isolation and characterization of catalase promoter from a filamentous fungus”; Dept of Biotechnology, Hamdard University, New Delhi (2009)