Dr. Asif Ali

Natural Product Chemistry Division
CSIR - Indian Institute of Integrative Medicine,Canal Road, Jammu – 180001
Email: asifali@iiim.ac.in

Positions Held  
Position Held Date Organization
Senior Scientist 2012 – Present CSIR-IIIM
Scientist Fellow 2010 – 2012 CSIR-IIIM
Postdoctoral Fellow 2009 – 2010 Max Planck Institute of Colloids and Interfaces, Germany
Sr. Research Fellow 2006 – 2009 Department of Biochemistry, National University of Singapore, Singapore
Principle scientist  2005 – 2006 Brain & Beyond Biotech. Pharmaceutical limited, Mysore, India.
Research Associate 2003 – 2005 National Institute of Immunology, New Delhi, India.
Technical Officer 2002 – 2003 Faculty of Pharmacy, Hamdard University, New Delhi, India
Membership  
  • Editorial board member of Journal of Antibiotic research

  • Reviewer of ACS, Science Direct Journal

Research

Total synthesis of antigenic Glycolipids:

Total synthesis of the phospholipomannan (PLM) which was found in cell wall of Candida albicans. It is most widespread human pathogen responsible for cutaneous and systemic fungal infections, synthesize a unique sequences of β-1,2-oligomannan on its cell surface that act as adhesion, induce production of pro-inflammatory cytokines and antibodies.
And it is highly immunogenic due to its β-1,2-mannan structural motif, which is absent in human host. The PLM is a strong TNF-α inducer in vivo and in vitro. The target molecule contain synthetic challenges such as an unusual β-1,2-oligomannan, Anomeric phosphodiester and phyto sphingosine linked to saccharide through phosphate.

Isolation of bioactive metabolites from Natural resources: we are focusing the several distinct scientific exploratory projects which is mainly related to isolation and characterization of bioactive metabolite from endophytic microbes, Medicinal plants and marine organism for new chemical entities to achieve the new biological knowledge.

Isolation of distinct metabolites from microbes: Phialomustin A-D (1-4), four new distinct bioactive metabolite with an unprecedented azaphilone derived skeleton form an endophytic fungus isolated from corms of Crocus sativus showed highest ITS-5.8S-ITS2 ribosomal gene sequence and morphological analysis sequence similarity more than 99% homology with Phialophora mustea.

Chemical modification and crystallization as an alternative source for the isolation of bioactive molecules from the endophytic fungus, Cryptosporiopsis sp.: Isolation of distinct metabolite from an endophyte of Clidemia hirta was revaluated of the ITS region displayed a 98% sequence similarity with Cryptosporiopsis californiae.

Marine Microbes: Isolation of distinct cyclic dipeptide from Streptomyces sp.:

Plants Metabolite: 3 new and several know distinct carbazole alkaloids isolated from the stem of Murraya koenigii.

 

Natural Product a versatile biochemical tools and their application: 
In addition to that we are keen on to emphasize how natural products or engineered analogs can be used as chemical tools to apprehend some biological problems and create small-molecule probes that interact with a specific protein in a broader biological knowledge for understanding of what makes a high-quality probe which is lacking. We would like to set up the distinct examples of natural products and their derivatives as biochemical tools to explore biological phenomena and assemble biochemical pathways are presented in Figure 1.

Medicinal chemistry: My research group are also actively involved in the semisynthetic modification of lead natural product to achieving the new biological knowledge.

Publications
  • Kumar, S.; Pathania, A. S.; Satti, N. K.; Dutt, P.; Sharma, N.; Mallik, F. A.; Ali, A. Synthetic modification of hydroxychavicol by Mannich reaction and alkyne-azide cycloaddition derivatives depicting cytotoxic potential. Eur. J. Med. Chem. (2015), 92, 236-245.

  • Kumar, S.; Pathania, A. P.; Nalli, Y.; , Malik, F. A.; Vishwakarma, R. A.; Ali, A. Synthesis of new O-alkyl and alkyne–azide cycloaddition derivatives of 4'-methoxy licoflavanone: a distinct prenylated flavonoids depicting potent cytotoxic activity. Med. Chem. Res. (2015), 24, 669-683.

  • Mahajan, V.; Sharma, N.; Kumar, S.; Bhardwaj, V.; Ali, A.; Khajuria, R. K.; Bedi, Y. S.; Vishwakarma, R. A.; Gandhi, S. G. Production of rohitukine in leaves and seeds of Dysoxylum binectariferum: An alternate renewable resource. Pharm Biol. (2015), 53, 446-450.

  • Ankita, M.; Yedukondalu, N.; Manoj, K.; Chand, R.; Ajai, P. G.; Ali, A.; Vishwakarma, R. A.; Chaubey, A.; An efficient liquid chromatography–tandem mass spectrometry method for the quantification of borrelidin from Streptomyces rochei (ATCC 10739).J. Chem. Technol. Biotechnol. (2014).

  • Zilla, M. K.; Nayak, D.; Amin, H, Nalli, Y.; Kitchlu, S.; Goswami, A.; Ali, A.4'-demethyl-deoxypodophyllotoxin glucoside isolated from Podophyllum hexandrum exhibits potential anticancer activities by altering Chk-2 signaling pathway in MCF-7 breast cancer cells.Chem. Biol. Inter. (2014), 224, 100-107.

  • Zilla, M. K.; Nayak, D.; Vishwakarma, R. A.; Sharma, P. J.; Goswami, A.; Ali, A. A convergent synthesis of alkyne–azide cycloaddition derivatives of 4-α,β-2-propyne podophyllotoxin depicting potent cytotoxic activity. Eur. J. Med. Chem.(2014), 77, 47-55.

  • Gannedi, V.; Ali, A.; Singh, P. P.; Vishwakarma, R. A. Intramolecular aglycon delivery for (1→2)-β-mannosylation: towards the synthesis of phospholipomannan of Candida albicans. Tet. Lett. (2014), 55, 2945–2947.

  • Zilla, M. K.; Qadri, M.; Pathania, A. P.; Strobel, G. A.; Nalli, Y.; Kumar, S.; Guru, S. K.; Bhushan, S.; Singh, S. K.; Vishwakarma, R. A.; Hassan, S. R.; Ali, A.Bioactive metabolites from an endophytic Cryptosporiopsis sp. inhabiting Clidemia hirta. Phytochemistry (2013), 95, 291-297.

  • Kumar, S.; Pathania, A. P.; Saxena, A. K.; Vishwakarma, R. A.; Ali, A.; Bhushan, S. Anticancer potential of flavonoids isolated from stem bark of Erythrina suberosa through induction of apoptosis and inhibition of STAT signaling pathway in Human leukemia HL-60 cells. Chem. Biol. Inter. (2013), 205, 128-137.

  • Jain, S.K.; Pathania, A.S.; Parshad, R.; Raina, C.; Ali, A.; Gupta, A.P.; Kushwaha, M.; Aravinda,S.; Bhushan, S.; Bharate, S.B.; Vishwakarma, R.A. Chrysomycins A-C, antileukemic naphthocoumarins from Streptomyces sporoverrucosus. RSC Adv. (2013), 3, 21046-21053.
  • Ali, A.; Khajuria, A.; Sidiq, T.; Kumar, A.; Thakur, N. L.; Naik, D.; Vishwakarma, R. A. Lawsonone immunosuppressive Lawsonyl monocyclic  terpene from the Marine-Derived Streptomyces sp. Immunology Lett. (2013), 150, 79-86.

  • Ali, A.; Vishwakarma R. A. Total synthesis of fully lipidated glycosylphosphatidylinositol anchor of Plasmodium falciparum. Tetrahedron, (2010), 66, 4357-4369.
  • Ali, A.; Wenk, M. R.; Lear, M. J. New Resolution Approach En Route to Phosphatidyl- myo-Inositol Mannoside Analogues of Mycobacterium tuberculosis. Tetrahedron Lett. (2009), 50, 5664-5666.

  • Ali, A.; DC Gownda, R A Vishwakarma. A new approach to construct full-length glycosylphosphatidylinositols of parasitic protozoa and [4-deoxy-Man-III]-GPI analogues. Chem. Commun. (2005),4, 519-521.

  • S. Alam, G. Kaur, A. Ali, H. Hamid, M. Ali, M. Athar.  Two new bioactive oleanane triterpene glycosides from Terminalia arjuna. Nat. Prod. Res. (2008),22, 285-294.

  • Ali, A.; Ali, M.; Alam, S. Two New Oleanane Triterpene Glycosides from the Bark of Terminalia arjuna Z. Naturforsch. (2006), 61b, 1282-1286.

  • Kaur, G.; Hamid, H.; Ali, A.;  Alam, M. S.; Athar, M. Anti inflammatory evaluation of Alcoholic extract of Galls of Quercus infectoria. J. Ethnopharmacol. (2004),90, 285-292.

  • Hamid, H.; Abdullah, S. T.; Ali, A.; Alam, M. S.; Ansari, S. H. Anti-inflammatory and analgesic activity of Uraria lagopoides. Pharmaceutical Biology(2004), 42, 114-116.

  • Ali, A.; kaur, G.; Abdullah, S. T.; Ali, M.; Alam, S.; Niwa, M. Terminoside A, a new triterpene glycosides from the bark of Terminalia arjuna inhibits nitric oxide production in murine macrophages. J. Asian Nat. prod. Res. (2003), 5, 137-42.

  • Abdullah, S. T.; Ali, A.; Hammid, H.; Ali, M.; Ansari, S. H.; Alam, M. S.; Two new anthraquinones from the roots of Rubia cordifolia Linn. Die Pharmazie, (2003),58, 216-217.
  • Ali, A.; Abdullah, S. T.; Hammid, H.; Ali, M.; Alam, M. S. A new sterol from the pseudobulb of Desmotrichum fimbriatum Blume. Die Pharmazie, (2003),58, 361-362.

  • Ali, A.; Abdullah, S. T.; Hammid, H.; Ali, M.; Alam, M. S. Phytochemical investigation of pseudobulb of Desmotrichum fimbriatum Blume. Ind. J. Pharm. Sci. (2003),208-211.

  • Ali, A.; Abdullah, S. T.; Hammid, H.; Ali, M.; Alam, M. S. Two new Petacyclic triterpene glycosides from the bark of Terminalia arjuna. Ind. J. Chem. Sec. (2003),42B, 2905-2908.

  • Ali, A.; Abdullah, S. T.; Hammid, H.; Ali, M. A new Picoline alkaloid from the pseudobulb of Desmotrichum fimbriatum Blume. Die Pharmazie, (2003),58, 716-20.

  • Ali, A.; kaur, G.; Ali, M. A novel naphthalene glycosides from Terminalia arjuna with antioxidant and nitric oxide inhibitory activity. Die Pharmazie, (2003), 58, 932-934.

 
Current Students

CSIR Fellow
1
Nalli Yedukondalu (SRF UGC)
2
Mahesh Kumar (SRF-UGC)
3
Murtaza Shah (JRF)
Facilities
1
LCMS, HRMS
2
NMR (400MHz, 500MHz)
3
HPLC
4
Flash chromatography
5
IR